The IVIS Spectrum scanner enables users to quantify depth, geometry and intensity for both bioluminescent and fluorescent sources in three-dimensional spatial resolutions. The IVIS Spectrum platform supports simultaneous molecular and anatomical longitudinal studies of disease progression, cell trafficking and gene expression patterns in living animals. The primary use of the instrumentation is to non-invasively monitor bioluminescence and fluorescence in three-dimensions within small animal models (rats and mice). The Xenogen IVIS Spectrum Instrument with Fluorescence Kit, Anaesthesia System, and Living Image Acquisition/Analysis Package provides the capacity to non-invasively scan up to 5 mice at one time with the capacity to monitor in vivo multiple fluorescent reporter and bioluminescent reporters simultaneously. The Living Image Acquisition/Analysis Software Package that is included with the system provides advanced quantification and analysis of all data acquired in three dimensions from each animal. The IVIS platform offers imaging solutions for a wide range of applications within biomedical research including oncology, infectious disease, musculoskeletal, cardiovascular, neurology, respiratory and stem cell research. Pharmacokinetics/pharmacodynamics studies and analysis are also possible using the IVIS Spectrum platform.
In case of fluorescence scanning it can be advisable to use diets without phytochromes, especially when scanning abdominal region. The fur of mice and rats are highly autofluorescent. An integrated software in the scanner can subtract the auto-fluorescence signal by ‘spectral unmixing’. For further information please see guides on the IVIS computer.