IVIS In Vivo Imaging System
IVIS Spectrum scanner
The IVIS Spectrum scanner enables users to quantify depth, geometry and intensity for both bioluminescent and fluorescent sources in three-dimensional spatial resolutions. The IVIS Spectrum platform supports simultaneous molecular and anatomical longitudinal studies of disease progression, cell trafficking and gene expression patterns in living animals. The primary use of the instrumentation is to non-invasively monitor bioluminescence and fluorescence in three-dimensions within small animal models (rats and mice). The Xenogen IVIS Spectrum Instrument with Fluorescence Kit, Anaesthesia System, and Living Image Acquisition/Analysis Package provides the capacity to non-invasively scan up to 5 mice at one time with the capacity to monitor in vivo multiple fluorescent reporter and bioluminescent reporters simultaneously. The Living Image Acquisition/Analysis Software Package that is included with the system provides advanced quantification and analysis of all data acquired in three dimensions from each animal. The IVIS platform offers imaging solutions for a wide range of applications within biomedical research including oncology, infectious disease, musculoskeletal, cardiovascular, neurology, respiratory and stem cell research. Pharmacokinetics/pharmacodynamics studies and analysis are also possible using the IVIS Spectrum platform.
Key features
- Integrated optical technology
- 3D optical tomography for fluorescence and bioluminescence
- Sensitive detection technology for:
- Bioluminescence
- Multispectral fluorescence and spectral unmixing
- Cerenkov imaging for optical radiotracer imaging
- DyCETM dynamic enhanced imaging for real time distribution studies of either fluorochromes or PET tracers ideal for PK/PD applications
Practical information concerning the IVIS Spectrum scanner
- Isoflurane usage is not included and neither are consumables such as isoflurane filters, black paper and nose cones.
- Please use another computer for data analysis. A computer for data analysis is available next to the scanner for example.
- Clean the instrument with water and ethanol. Never with virkon (red stuff) as it damages the surfaces of the instrument and increases background fluorescence.
- Do not spray with liquids inside the instrument as there are light sources and electrical components under the platform. In addition, you do not want liquid particles on the camera lens.
- Avoid leaving liquids in the instrument, which can evaporate and condense on the camera lens.
- Never shut down the computer when you leave, but turn off the screen. The instrument can only calibrate during the night if the computer is on.
- Remember to refill isoflurane for the next user and note isoflurane use.
- Used isoflurane filters for disposal should be delivered to Ole Frandsen, at the Health department.
Notes concerning auto fluorescence
In case of fluorescence scanning it can be advisable to use diets without phytochromes, especially when scanning abdominal region. The fur of mice and rats are highly autofluorescent. An integrated software in the scanner can subtract the auto-fluorescence signal by ‘spectral unmixing’. For further information please see guides on the IVIS computer.