Objective | Mag. / NA | Medium |
Standard, Olympus UPLXAPO100 | 100x/1.45, WD 0.13 mm | Oil |
Lasers | Type and Power | Selected dyes |
405 nm | Diode, 1 W | DAPI, Hoechst 33342 |
488 nm | Diode, 1 W | Alexa488, Atto488, CF488A |
561 nm | DPSS, 500 mW | Alexa 555, dylight 555, CF568 |
640 nm | Diode, 1 W | Alexa 647 |
Imaging modalities:
Achievable resolution:
3D imaging technique: Astigmatism
Camera: Latest generation sCMOS 82 % peak QE, 1.6 electrons rms read noise at standard scan
sCMOS camera: Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera
Time for super-resolution full frame: Seconds to minutes (number of localizations and laser power dependent)
Simultaneous imaging channels: 1 or 2
Total number of imaging colors: Up to 4 lasers
Field of view: 100 μm x 100 μm (1 channel) or 100 μm x 50 μm (2 channels)
Other light sources: LED for bright-field imaging, NIR auto-focus laser
Emission Channels:
Illumination modes: Closed-loop, continuous illumination angle adjustment between epi-illumination and total-internal reflection
Sample stage: 18/18/9 mm XYZ travel range, closed-loop piezo stage with 2 nm encoder resolution
Temperature control (live cell imaging): Resistive heating, whole instrument
Focus system: One-shot autofocus; Continuous autofocus
Mechanical stability: <1 μm/K drift; <1 nm vibration amplitude (1 Hz to 500 Hz)