This Light Sheet Microscopy Workshop brings together young scientists and experts in the field of light sheet microscopy.
We aim to give you an overview on current best practice in light sheet microscopy along with tips & tricks for every step from methodological aspects all the way to data analysis and to facilitate increased awareness of both the prospects and the limitations of light sheet microscopy.
The workshop is intended for PhD students and postdocs engaged or interested in light sheet microscopy.
Please note that you are not guaranteed a seat at the workshop, if you do not meet the target group requirements. Registrations will be considered in the order in which they are received.
Or do you have questions or problems? Reach out to us in our contact form.
Workshop fee: 3,000 DKK
Light sheet microscopy offers intermediate-to-high optical resolution, good optical sectionning capabilities and high speed - perhaps the right choice for your research? Designing a proper experimental setup that fits your experiment may be challenging and may require a lot of time. This workshop will take you through each step from methodological aspects to data analyses:
Altogether, our aim is to give you an overview on current best practice in light sheet microscopy, to provide tips and tricks on each step and to ensure that you obtain basic light sheet microscopy experience by performing each step yourself. Finally, there will be the possibility to present a poster.
Experts will be happy to answer your questions along the way!
Participants should bring their own laptop (with mouse for convenience) for the Arivis data anlysis part. It is necessary to remote connect to the Acquifer Hive system where Arivis runs. If you would like to take your data home with you, bring a large hard drive (min 1TB).
To make optimal use of the workshop, bring your own sample. For support, contact us before you start clearing (at least 3 weeks before the beginning of the workshop). Contact us at imaging@biomed.au.dk
Day 1 | 10:30 - 17:00 + Welcome Reception 18:00 - 20:00 |
Day 2 | 09:00 - 17:30 |
Day 3 | 09:00 - 17:30 |
Day 4 | 09:00 - 13:00 |
10:30 - 11:00 | Welcome & Introduction |
11:00 - 11:30 | Theoretical session |
11:30 - 12:30 | Theoretical session |
12:30 - 13:30 | Lunch break |
13:30 - 14:30 | Theoretical session |
14:30 - 17:00 | Sessions |
18:00 - 20:00 | Poster Presentations & Welcome get-together |
09:00 - 09:30 | Hands-on tissue clearing |
09:30 - 10:30 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
10:30 - 11:00 | Coffee break |
11:00 - 12:00 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
12:00 - 13:00 | Lunch break |
13:00 - 14:30 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
14:30 - 15:00 | Coffee break |
15:00 - 16:00 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
16:00 - 16:30 | Afternoon snack |
16:30 - 17:30 | Theoretical sessions |
09:00 - 09:30 | Theoretical session |
09:30 - 10:30 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
10:30 - 11:00 | Coffee break |
11:00 - 12:00 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
12:00 - 13:00 | Lunch break |
13:00 - 13:30 | Theoretical sessions |
13:30 - 14:30 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
14:30 - 15:00 | Coffee break |
15:00 - 16:00 | Parallel sessions on Zeiss Lighsheet7, Flamingo (RG Huisken) and Arivis |
16:00 - 16:30 | Afternoon snack |
16:30 - 17:30. | Theoretical session |
09:00 - 11:00 | Talks: - TBA - TBA Optional: run last analysis scripts (start in the morning) |
11:00 - 12:00 | Group presentations |
12:00 - 13:00 | Lunch break & Farewell |
Clearing & Imaging
Jacques Paysan | Application specialist, Zeiss |
Maria Trullson | Application specialist, Zeiss |
Mostafa Aakhte | PostDoc, Huisken Lab |
Steven Edwards | Light sheet microscopy expert, SciLifeLab, Royal Institute of Technology (KTH) |
Big Data Storage & Analysis
Olaf Selchow | Consultant, Acquifer (Bruker) |
David Wiles | Senior Application Engineer, ZEISS arivis |
Molly McQuilken | Business Development Software Solutions, ZEISS arivis |
Invited Speakers
TBA |
Signing up
When signing up, you will be asked for EAN or CVR numbers. For the correct numbers, please consult the person in your lab in charge of invoicing and finances. These are unique numbers for your university/institution: EAN (European Article Number) used for digital invoicing, CVR (Central Business Register) used to identify businesses. In stead of a CVR number, a VAT number may be used, if your institution is VAT registered.
Poster presentation & Welcome Reception
Please bring a poster on the 19th of August 2024 where the The Bioimaging Core Facility organises poster presentations along with informal catering. Participation is free of charge.
Poster dimensions: A0 upright are preferred
Practicalities
Please bring your own laptop every day.
Accomodation & Venue
> Accommodation will need to be organised by each participant. We are unfortunately unable to provide accomodation.
> Aarhus is a fairly small city with most accomodations within walking distance.
> Public transportation in Aarhus
> Aarhus University Campus Map (in DK only; type in the building number/name in the search field)
> For Aarhus University app, download AU Find for iOS or Android
Certification
A workshop certificate will be sent by e-mail to participants, upon request, after the workshop.
Zeiss, Acquifer (Bruker)