AxioObserver (Bartholin - 1242), Inverted Widefield, Zeiss

Pancreatic tumour cells, César Rodriguez Santana

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Applications in brief

This inverted widefield microscope is equipped with two light sources and two corresponding detectors (cameras). This allows generation of either fluorescence images or brightfield imagages.

In doubt if your staining worked? Book a 15 min time slot to check your sample.

Features

Brightfield and/or multispectral fluorescence
Stage mounting frames for slides (max. length 120 mm) and petri dishes (Ø 24-68 mm)

Which samples may I use?

Selected samples, but not limited to:

Fixed tissue sections or fixed cells
Brightfield (e.g. H&E)
Immunofluorescence

Specifications

Full equipment name	Widefield Inverted Light Microscope, Zeiss
Objectives	10x/0.25, Air, LongDistance A-Plan, coverslip thickness: 1 mm polystyrene 40x/0.75, Air, EC Plan-Neofluar, coverslip thickness: 0.17 mm 63x/1.40, Oil, Plan Apochromat, coverslip thickness: 0.17 mm
Filter qubes	Cy 5 (EX BP 640/30, BS FT 660, EM BP 690/50) Cy3 (EX BP 550/25, BS FT 570, EM BP 605/70) eGFP (EX BP 470/40, BS FT 495, EM BP 525/50) Hoechst/DAPI (EX BP 390/40, BS FT 420, EM BP 450/40)
Light sources	Illuminator microLED (for brightfield applications) Colibri 5 type RGB-UV (for fluorescence applications)
Detectors (cameras)	Axiocam 506 mono (for fluorescence applications) Axiocam 105 color R2 (for brightfield applications)
Heated stage	No
Motorized stage	No

Location

building 1242
room 156

New user?

Booking

Pricing

Need help?

Come to our Open Office sessions to discuss your imaging needs or get advice on workflows, free of charge:

Tuesdays and Thursdays 13:30 -14:30

The Skou building, 1116-256

Revised 07.08.2026

Nina Glöckner Burmeister