Slide Scanner, Upright Widefield, Olympus

Would you like your image to be displayed here?

Please contact us or send an email to imaging@biomed.au.dk, if you would like to share your images - Thank you

The VS120 Slide Scanner from Olympus is convenient for automated imaging of tissue sections located on several slides.

Applications in brief

This virtual 6-slide scanning system creates high resolution images in brightfield (all magnifications), darkfield (4x, 10x, and 20x) and / or multi-fluorescence mode (all magnifications). Image stitching is very precise enabling high-level accuracy that can be applied from small animal brain slices to large specimens. Seamless switching between micro and macro observation modes enable swift viewing of regions of interest and overall structures.

Features

Brightfield, darkfield, and widefield fluorescence scanning
Color corrected camera for brightfield/darkfield scanning
Darkfield mode to increase contrast in unstained samples
Fast and powerful light engine with single-band exiters and a fast filter wheel with single-band emitters for DAPI, FITC, Cy3, Cy5 and Cy7

Which samples may I use?

Selected samples, but not limited to:

tissue sections
up to 6 slides simultaneously

Training and use

Specifications

Full equipment name	VS120 Slide Scanner, Olympus
Objectives	2x / NA 0.08 10x / NA 0.4 20x / NA 0.75 40x / NA 0.95
Channels	DAPI: EX 395/25; 295mW, EM 425/50 FITC: EX475/28; 196mW, EM 525/50 Cy3: EX 575/25; 310mW, EM 600/60 Cy5: EX 635/22; 231mW, EM 680/80 Cy7: EX 730/40, EM 800/100
Filter	DAPI/FITC/Cy3/Cy5/Cy7 Pentafilter Semrock, Penta LED HC Filter Set #F68-050)
Cameras	Monochrome camera: Hamamatsu ORCA-FLASH4.0 V2 (QE 82%) Color camera: AVT Pike F-505C VC50 progressive scan CCD camera
Slide capacity	up to 6 slides simultaneously