Lightsheet 7, Zeiss

Light sheet 7

  • Dual-side light sheet microscope
  • Multiview
  • Imaging of Living and Cleared Specimens
  • 5x, 10x and 20x detection objectives

Specifications

2 cameras

Detection: pco.edge 4.2 cooled sCMOS camera

  • 0.8 e- med (median) readout noise
  • 2048 x 2048 pixel resolution
  • up to 82 % quantum efficiency

Location:

The Skou building 1116, room 550


Laser lines: 

405 nm        50 mW

488 nm        50 mW

561 nm        50 mW

640 nm        75 mW

Objective Mag. / NA Medium Contrast Working Distance (mm)

Filter modules:

LBF 405/488/561/638

  • DAPI-GFP (LP 490 and two emission filters BP 420-470 and BP 505-545)
  • GFP-Cy3 (LP 560 and two emission filters BP 505-545 and BP 575-615)
  • Cy3-DRAQ5 (LP 640 and two emission filters BP 575-615 and LP 660)
  • LP 580
  • LP 640

Illumination Optics:

  • 2 each for forming the lightsheet
  • Illumination optics 5x/0.1 foc
  • Illumination optics 10x/0.2 foc

Imaging chambers:

  • Starterkit sample chamber+sample holder
  • Sample chamber set f/clearing 20x
  • Sample chamber set f/large samples