LSM 800, Airyscan, Laser Scanning Confocal with Superresolution, Zeiss

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The LSM 800 inverted laser scanning confocal microscope from Zeiss is equipped with an airyscan detector, increasing sensitivity and resolution.

Applications in brief

The LSM 800 inverted confocal laser scanning microscope is equipped with a highly sensitive GaAsP detector and performs fast linear scanning. Its Airyscan detector acquires optical sections with a resolution of 120 nm (in x,y) and 350 nm (in z). With the 4-8 times better signal-to-noise ratio (SNR) of Airyscan, you will be pushing sensitivity and resolution.

Features

3 detectors
Airyscan detector
1 transmitted detector

Which samples may I use?

Selected samples, but not limited to:

fixed cells
fixed tissue

Training and use

Specifications

Full equipment name

LSM800 inverted laser scanning confocal microscope with Airyscan, Zeiss

Objective	Mag. / NA	Medium	Contrast	Working Distance (mm)
Pln Apo	5x/0,16	Air	-	12,1
Pln Apo	10x/0,45	Air	DICII	2
Pln Apo	40x/0,95	Air	DICIII	0,25

Pln Apo	63x/1,4	Oil	DIC III	0,19

Lasers	Type and Power	Selected dyes
405nm	Diode	DAPI, Hoechst
488nm	Diode	GFP, AlexaFluor488
561nm	Diode	CY3
640nm	Diode	CY5

FAQ

Equipment

- How should I clean the equipment after use?

Please leave the work station neat and clean for the next user.

- Are consumables included in the price?

Experimental consumables are not included in the price for booking of microscopes via the Bioimaging Core Facility.
Designated oil for the oil objectives can be found next to the microscope. Please only use this designated oil for the objectives.
Lens paper designated for cleaning of objects as well as designated tissues for cleaning of samples are located next to the microscope.

Data Storage

- How can I get access to my data?

After using a microscope or a workstation, please transfer your data immediately to your personal AU drive (O:) and clean up your local directories on instrument PCs if you are not reusing data in subsequent experiments.
The Bioimaging Core Facility staff will provide support to non-AU users for exporting data.
Use of USB memory sticks or external hard drives is for security reasons not allowed.

- Does the Bioimaging Core Facility regularly back up the user data?

The Bioimaging Core Faility is not responsible for your data. DATA ON LOCAL PC DRIVES WILL BE DELETED ON A REGULAR BASIS in order to keep the systems performing optimally.

Please ensure that you transfer your data to your personal AU drive (O:) after each session.

- May I use my personal USB memory stick or external hard drive for data storage?

No, any use of USB memory sticks or external hard drives is for security reasons not allowed. Usage of USB memory sticks may lead to suspension of the user license.