The motorized nosepiece automatically focuses onto the sample. The equally motorized stage can either be positioned by hand, enabling rapid gross sample alignment, or it can be encoded to continuously read-out the X and Y position, enabling the user to set precise coordinates and to navigate directly to them.
Selected samples, but not limited to:
My section has varying thickness or is mounted unevenly. How do I get a crisp image?
Please check all z-heights of focus points before starting the acquisition --> danger of damaging objectives if not correctly set up
Full equipment name | BX63 Upright Widefield Fluorescence Microscope, Olympus |
Camera | Sensitive Andor Zyla 5.5 (5 Megapixel, 6.5 µm pixel – 60% Quantum efficiency) |
Objectives |
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Filter qubes |
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Light source |
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The Bioimaging Core Faility is not responsible for your data. DATA ON LOCAL PC DRIVES WILL BE DELETED ON A REGULAR BASIS in order to keep the systems performing optimally.
Please ensure that you transfer your data to your personal AU drive (O:) after each session.
No, any use of USB memory sticks or external hard drives is for security reasons not allowed. Usage of USB memory sticks may lead to suspension of the user license.
* Deconvolution is an image processing technique used to improve contrast and resolution of images. Out-of-focus light causes blur, which mathematically is represented as a convolution operation. Deconvolution seeks to reassign out-of-focus light to the correct origion. Nearly all fluorescence images can be deconvolved.