Spinning Disk Confocal, Olympus
Olympus IX83 with spinning disk confocal
Fast time-lapse confocal imaging of living cells with minimal phototoxicity and less photobleaching. Stability during long-term and high-speed imaging facilitates quantitative analysis of huge amounts of data
Specifications
- 4 line laser engine: iChroms MLE 405nm, 488nm, 561nm, 640nm
- Camera: Hamamatsu ORCA-FusionBT sCMOS (formerly: Orca Fusion BT C15440)
- Environment control: cellVivo CO2 and heat control
- Objectives: 40x (Oil), 60x (Water), 100x (Oil), and 100x (Silicon oil)
- Z drift compensation system
Fluorescence filters
- Band pass filters (Confocal imaging)
- Quad band filter: 440-40 / 521-21 / 607-34 / 700-45
- Band pass filter: 525-50
- Band pass filter: 600-32
- Band pass filter: 625-90
- Band pass filter: 680-42
- Filter cubes (Widefield ocular observation)
- Triple band: Dapi/FITC/TRITC
- Blue single band: Exciter filter: 360-370 nm, beam splitter: 410 nm, barrier filter: 420-460 nm
- Green single band: Exciter filter: 470-495 nm, beam splitter: 505 nm, barrier filter: 510-550 nm
- Red single band: Exciter filter: 565-585 nm, beam splitter 595, barrier filter: 600-690
- Triple band: Dapi/FITC/TRITC
Fluorescence filters
