The LSM 710 can be used for a wide array of imaging applications on both live and fixed samples including multicolor imaging, protein co-localization, 3D z-stacks, and stitching as well as for time-lapse analysis for following cellular events in live samples.
Selected samples, but not limited to:
Full equipment name | LSM710 inverted laser scanning confocal microscope, Zeiss |
Objective | Mag. / NA | Medium | Contrast | Working Distance |
Pln-Apo | 20x/0.8 | Air | - | 0,55 |
C Apochromat | 40x/1,2 | Water | DIC III | 0.28 at cover glass 0.17 |
Plan-Apochromat | 63x/1.4 | Oil | DIC III | 0.19 |
Lasers | Type and Power | Selected dyes |
405nm | Diode, 30mW | DAPI, Hoechst 33342 |
458nm | Argon, 25mW | CFP |
488nm | Argon, 25mW | GFP, AlexaFluor488 |
514nm | Argon, 25mW | YFP |
543 nm | HeNe, 5mW | RFP |
633nm | HeNe, 5mW | Cy5, AlexaFluor648 |