LSM 710, Laser Scanning Confocal, Zeiss

   

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The LSM 710 inverted laser scanning confocal microscope from Zeiss allows optical Z-sectioning of various fluorophores in living specimens and fixed samples.

Applications in brief

The LSM 710 can be used for a wide array of imaging applications on both live and fixed samples including multicolor imaging, protein co-localization, 3D z-stacks, and stitching as well as for time-lapse analysis for following cellular events in live samples.

Features

  • Motorized XY-stage
  • Temperature control
  • 3 detectors
  • 1 transmitted detector

Which samples may I use?

Selected samples, but not limited to:

  • living specimens
  • fixed cells
  • fixed tissue

Specifications

Full equipment name LSM710 inverted laser scanning confocal microscope, Zeiss
Objective Mag. / NA Medium Contrast Working Distance
Pln-Apo 20x/0.8 Air - 0,55
C Apochromat  40x/1,2 Water
DIC III  
0.28 at cover glass 0.17
Plan-Apochromat 63x/1.4 Oil DIC III 0.19
Lasers           Type and Power  Selected dyes                       
405nm Diode, 30mW DAPI, Hoechst 33342
458nm Argon, 25mW CFP
488nm Argon, 25mW GFP, AlexaFluor488
514nm Argon, 25mW YFP
543 nm HeNe, 5mW RFP
633nm HeNe, 5mW Cy5, AlexaFluor648

Need help?

Come to our Open Office sessions to discuss your imaging needs or get advice on workflows, free of charge:

Tuesdays and Thursdays 13:30 -14:30

The Skou building, 1116-256