LSM 710, Laser Scanning Confocal, Zeiss

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The LSM 710 inverted laser scanning confocal microscope from Zeiss allows optical Z-sectioning of various fluorophores in living specimens and fixed samples.

Applications in brief

The LSM 710 can be used for a wide array of imaging applications on both live and fixed samples including multicolor imaging, protein co-localization, 3D z-stacks, and stitching as well as for time-lapse analysis for following cellular events in live samples.

Features

Motorized XY-stage
Temperature control
3 detectors
1 transmitted detector

Which samples may I use?

Selected samples, but not limited to:

living specimens
fixed cells
fixed tissue

Training and use

Specifications

Full equipment name

LSM710 inverted laser scanning confocal microscope, Zeiss

Objective	Mag. / NA	Medium	Contrast	Working Distance
Pln-Apo	20x/0.8	Air	-	0,55
C Apochromat	40x/1,2	Water	DIC III	0.28 at cover glass 0.17
Plan-Apochromat	63x/1.4	Oil	DIC III	0.19

Lasers	Type and Power	Selected dyes
405nm	Diode, 30mW	DAPI, Hoechst 33342
458nm	Argon, 25mW	CFP
488nm	Argon, 25mW	GFP, AlexaFluor488
514nm	Argon, 25mW	YFP
543 nm	HeNe, 5mW	RFP
633nm	HeNe, 5mW	Cy5, AlexaFluor648

FAQ

Equipment

- How should I clean the equipment after use?

Please leave the work station neat and clean for the next user.
Please ensure that you have removed any oil from the oil objective.
In case of accidental contamination of air/water objectives with oil, please contact the bioimaging core facility staff immediately.

- Are consumables included in the price?

Experimental consumables are not included in the price for booking of microscopes via the Bioimaging Core Facility.
Designated oil for the oil objectives can be found next to the microscope. Please only use this designated oil for the objectives.
Lens paper designated for cleaning of objects as well as designated tissues for cleaning of samples are located next to the microscope.

Data Storage

- How can I get access to my data?

After using a microscope or a workstation, please transfer your data immediately to your personal AU drive (O:) and clean up your local directories on instrument PCs if you are not reusing data in subsequent experiments.
The Bioimaging Core Facility staff will provide support to non-AU users for exporting data.
Use of USB memory sticks or external hard drives is for security reasons not allowed.

- Does the Bioimaging Core Facility regularly back up the user data?

The Bioimaging Core Faility is not responsible for your data. DATA ON LOCAL PC DRIVES WILL BE DELETED ON A REGULAR BASIS in order to keep the systems performing optimally.

Please ensure that you transfer your data to your personal AU drive (O:) after each session.

- May I use my personal USB memory stick or external hard drive for data storage?

No, any use of USB memory sticks or external hard drives is for security reasons not allowed. Usage of USB memory sticks may lead to suspension of the user license.