Slide Scanner, Upright Widefield, AxioScan 7, Zeiss
The AxioScan 7 Slide Scanner from Zeiss is convenient for automated imaging of tissue sections located on several slides.
Applications in brief
The AxioScan 7 slide scanner has a capacity of loading 100 standard-sized slides at the same time. The widefield scanning system creates high resolution images in brightfield and multi-fluorescence for all magnifications. Precise image stitching for all magnification objectives makes screening of small animal brain slices to large specimens fast and convenient. Seamless switching between micro and macro observation modes enable swift viewing of regions of interest and overall structures. Identified regions can be saved and loaded into the LSM900 in the neighbouring booth for confocal or super-resolution imaging.
Features
- Upright widefield system with high precision stitching
- Brightfield and multi-fluorescence imaging
- Color corrected camera for bright field imaging
- Fast and powerful light engine with single-band exiters and a fast filter wheel with single-band and multi-bandpass filter cubes covering e.g. DAPI, FITC, Cy3, Cy5 and Cy7
System specifications:
Objectives:
Objective N-Achroplan 5x/0.15 Pol M27 (WD=12.0mm)
Objective Plan-Apochromat 10x/0.45 M27 (WD=2.1mm)
Objective Plan-Apochromat 20x/0.8 M27 (WD=0.55mm)
Objective Plan-Apochromat 40x/0.95 Corr M27 (WD=0.25mm)
Excitation light source:
Viluma 7
(see picture below)
BP 385/30, BP 423/44, BP 469/38, BP 508/20, BP 555/30, BP 591/27, BP 631/33, BP735/40
- Fresh-up on convention on bandpass (BP) filter specs:
- BP Centerwavelength (nm) / TotalWidt (nm)
- example: BP 385/30 means 370-400nm
Filter cubes:
Filter set number | Name | Excitation filter | Beamsplitter | Emission filter |
01 | DAPI | BP 365/12 | BS 395 | LP 397 |
09 | Alexa Fluor 488 | BP 450-490 | BS 510 | LP 515 |
38 | HE eGFP | BP 470/40 | BS 495 | BP 525/50 |
43 | HE DsRed | BP 550/25 | BS 570 | BP 605/70 |
50 | Cy 5 | BP 640/30 | BS 660 | BP 690/50 |
110 | HE DAPI7GFP/Cy3.5/Cy7 | BP 385/20 BP 469/28 BP 591,5/17 BP 735/30 | BS 405 BS 493 BS 611 BS 762 | BP 425/30 BP 524/46 BP 634/32 BP 785/30 |
115 | HE Cy7 | BP 710/80 | BS 760 | BP 713,5/83 |
118 | HE YFP | BP 507,5/15 | BS 760 | BP 555/40 |
121 | mCherry | BP 555/20 | BS 583 | BP 640/80 |
Cameras:
Camera Axiocam 705 color R2
- 60 frames per second at full 5-megapixel resolution
Camera Axiocam 712 mono R2
- 20 frames per second in full 12-megapixel resolution
Slide capacity:
100 standard sized sized slides in one run possible.
25 loading slots for Mounting frames:
- Mounting frame for 4 slides 26x76mm (standard sized slides)
- Mounting frame for 2 slides 52x76mm
- Mounting frame for 1 slide 102x76mm
- Insert for slides 28x48mm
Viluma 7 light source bandpass filters
