Stellaris 8, Laser Scanning Confocal with Tau mode, Leica
Specifications
Incubation chamber:
Okolab, Temperature, CO2, Humidity control
Fluorescence excitation:
405nm laser line, e.g. for FRAP or photostimulation, 50mW output power
488nm laser line, e.g. for FRAP, 20mW output power
White light laser: tunable pulsed laser from 440-790nm with 1nm steps and pulse frequency of 78 MHZ
- 8 channel AOTF (AcoustoOpticalTunableFilter) for rapid modulation of laser intensity. Useful e.g. for FRAP
- Tau sense functionality: TauGating, TauContrast, TauSeparation, TauScan, GateScan
- Delivers pulsed excitation for FALCON (FAst LifetimeCONtrast)
- Allows recording of twodimensional excitation-emission spectra supported by software for setup and display of 2D spectra
- Power output:
- 440 nm > 1.1 mW
- 488 nm > 1.6 mW
- 560 nm > 2.0 mW
- 630 nm > 2.6 mW
- 790 nm > 3.5 mW
LEDs for ocular view: LED3 solidstate light source offering white light in the 390-680 nm range
- Filter cube "405": excitation BP405/60, BS 455, emission BP 470/40
- Filter Cube "FITC": excitation BP480/40, BS __, emission BP 527/30
- Filter cube "RHOD": excitation BP546/10, BS __, emission BP 585/40
Scanhead:
8kHz Tandem Scanner, switchable between resonant and non-resonant mode
Hardware zoom: stepless: 0.75x - 48x or in resonant mode 1.25x - 48x
Scan field rotation: 200° (KoenigRotator)
A) Non-resonant mode:
- speed of 1-2600 Hz in steps of 1 Hz,
- X2Y-Scanner design for homogeneous illumination in a large field of view (FOV)
- Up to 10 frames / second , 512 x 512
- Up to 131 frames / second , 512 x 16
- Max. Image resolution 8192 x 8192 or 64 Mpixels
- Line frequency up to 5200 Lines / second
- Large scanfield: 22 mm (FOV) diagonal in the intermediate image plane
B) Resonant mode:
- Feedback monitoring of the scanner for constant image quality
- Line frequency 16000 Hz (bidirectional)
- Up to 28 frames / second at 512 x 512 (full frame)
- Up to 290 frames / second at 512 x 16
- Scan format max.: 2496 x 2496 pixels with up to 3 channels simultaneously
- FRAP Zoomer for fast photobleaching experiments
- Scan field: 13 mm diagonal
Objectives:
HC PL APO 10x/0.40 CS2, Air, WD 2.56 mm
HC PL APO 20x/0.75 CS2, Air, WD 0.62 mm
HC PL APO 40x/1.25, motCORR CS2, GLYC, WD 0.35 mm
HC PL APO 63x/1.40 CS2, OIL, WD 0.14 mm
Stage:
Motorized stage with Super Z Galvo
AFC (Adaptive Focus Control) maintains a userspecified focus plane constant over time
Detection:
Free spectral detection with Acousto Optical Beam Splitter (AOBS)
- 3x HyD S detector: Detection range 410-850 nm, spectral tuning resolution 1 nm, minimal detection range 5 nm
- Typical photon detection efficiency > 58% @ 500nm.
- Multipixel silicon photomultiplier array.
- Extended dynamic range for maximal contrast and flexibility
- Analog, reflection and photon counting detection modes
- Power counting: 0,1,2 (double) photons can be identified
- enables TauSense: TauGating (up to 16 tunable timegates, simultaneously), TauContrast (mean photon arrival time), TauSeparation (lifetime based component separation)
- 1x HyD X detector: Detection range 410-850 nm, spectral tuning resolution 1 nm, minimal detection range 5 nm
- Increased sensitivity in red and far-red part of spectrum
- Specialised for fast FALCON in the visible range up to farred
- Very low dark noise for quantitative measurement methods like FLIM
- Features of HyD S (above)
- Transmitted light detector: PMT allowing interference conrast, phase contrast or birght field
Add-ons:
High speed FRAP capabilities with FRAP wizard
FRET acceptorphotobleaching (AB) wizard
FRET sensitized emission (SE) wizard
