Spinning Disk Confocal, Olympus

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Applications in brief

The IX83 inverted spinning disk confocal microscope allows fast time-lapse confocal imaging of living cells with minimal phototoxicity and less photobleaching. Stability during long-term and high-speed imaging facilitates quantitative analysis of huge amounts of data.

Features

Environment control (cellVivo CO₂ and temperature control)
Z-drift compensation system

Which samples may I use?

Selected samples, but not limited to:

fixed cells
fixed tissue

Training and use

Specifications

Full equipment name	IX83 inverted spinning disk confocal microscope, Olympus
Laser	4 line laser engine: iChroms MLE 405nm, 488nm, 561nm, 640nm
Camera	Hamamatsu ORCA-FusionBT sCMOS (formerly: Orca Fusion BT C15440)
Environment control	cellVivo CO2 and temperature control

Objectives

Mag./NA	Medium
40x	Oil
60x	Water
100x	Oil
100x	Silicon oil

Fluorescence filters

Band pass filters (Confocal imaging)	Quad band filter: 440-40 / 521-21 / 607-34 / 700-45
	Band pass filter: 525-50
	Band pass filter: 600-32
	Band pass filter: 625-90
	Band pass filter: 680-42
Filter cubes (Widefield ocular observation)	Triple band: Dapi/FITC/TRITC
	Blue single band: Exciter filter: 360-370 nm, beam splitter: 410 nm, barrier filter: 420-460 nm
	Green single band: Exciter filter: 470-495 nm, beam splitter: 505 nm, barrier filter: 510-550 nm
	Red single band: Exciter filter: 565-585 nm, beam splitter 595, barrier filter: 600-690

Explainer: Fluorescence filters

FAQ

Equipment

- How should I leave the equipment for the next user?

Please familiarize yourself with the last part of the above 'Guide to calibrate Z-limit for different stages' in the pull-down section 'Training and use'.

- How should I clean the equipment after use?

Please leave the work station neat and clean for the next user.

- Are consumables included in the price?

Experimental consumables are not included in the price for booking of microscopes via the Bioimaging Core Facility.
Designated oil for the oil objectives can be found next to the microscope. Please only use this designated oil for the objectives.
Lens paper designated for cleaning of objects as well as designated tissues for cleaning of samples are located next to the microscope.

Data Storage

- How can I get access to my data?

After using a microscope or a workstation, please transfer your data immediately to your personal AU drive (O:) and clean up your local directories on instrument PCs if you are not reusing data in subsequent experiments.
The Bioimaging Core Facility staff will provide support to non-AU users for exporting data.
Use of USB memory sticks or external hard drives is for security reasons not allowed.

- Does the Bioimaging Core Facility regularly back up the user data?

The Bioimaging Core Faility is not responsible for your data. DATA ON LOCAL PC DRIVES WILL BE DELETED ON A REGULAR BASIS in order to keep the systems performing optimally.

Please ensure that you transfer your data to your personal AU drive (O:) after each session.

- May I use my personal USB memory stick or external hard drive for data storage?

No, any use of USB memory sticks or external hard drives is for security reasons not allowed. Usage of USB memory sticks may lead to suspension of the user license.