Department of Biomedicine

Bioimaging Core Facility

 Services and Equipment Lightsheet 7, Zeiss

Lightsheet 7, Zeiss

Light sheet 7

  • Light-Sheet Multiview Imaging of Living and Cleared Specimens
  • Observe real life – fast and sensitively.
  • Image large specimens in your preferred clearing solution.
  • Get best image quality for various applications.

Specifications

Detectors:

Detection: pco.edge 4.2 cooled sCMOS camera

  • 0.8 e- med (median) readout noise
  • 2048 x 2048 pixel resolution
  • up to 37 500 : 1 dynamic range
  • 100 fps maximum frame rate
  • up to 82 % quantum efficiency
  • Camera Link HS or USB 3.0

Location:

The Skou building 1116, room 550

Book

Lasers

Type and Power

Selected dyes

405nm

 Solid state laser (50mW)

DAPI, Hoechst 33342

488nm

 Solid state laser (50mW)

GFP, AlexaFluor488

561nm

 Solid state laser (50mW)

Cy3, Alexa 555

640nm

 Solid state laser (75mW) DRAQ5, Alexa 635
Objective Mag. / NA Medium Contrast Working Distance (mm)

Filter modules:

LBF 405/488/561/638, including a notch filter to block the lines 405, 488, 561 and 638nm as well as an anti-reflection coated 2 mm compensation glass.

For dual channel imaging:

  • DAPI-GFP (SBS LP 490 and two emission filters BP 420-470 and BP 505-545)
  • GFP-Cy3 (SBS LP 560 and two emission filters BP 505-545 and BP 575-615)
  • Cy3-DRAQ5 (SBS LP 640 and two emission filters BP 575-615 and LP 660)
  • empty with SBS LP 580
  • empty with SBS LP 640

Illumination Optics:

  • 2 each for forming the lightsheet
  • Illumination optics 5x/0.1 foc
  • Illumination optics 10x/0.2 foc

Imaging chambers:

  • Starterkit sample chamber+sample holder
  • Sample chamber set f/clearing 20x
  • Sample chamber set f/large samples
Revised 25.01.2024
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Nina Glöckner Burmeister