AxioObserver (Anatomy), Inverted Widefield, Zeiss

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The AxioObserver (Anatomy) Inverted Widefield holds a motorised stage and is easy to use while maintaining sensitivity.

Applications in brief

This inverted widefield microscope is equipped with two light sources and two corresponding detectors (cameras). This allows generation of either fluorescence images or brightfield imagages.

In doubt if your staining worked? Book a 15 min time slot to check your sample.

Features

  • Brightfield and/or multispectral fluorescence
  • Stage mounting frames for slides (max. length 120 mm) and petri dishes (Ø 24-68 mm)

Which samples may I use?

Selected samples, but not limited to:

  • fixed tissue sections or fixed cells
  • brightfield (e.g. H&E)
  • immunofluorescence

Price structure

Fixed Price Structure for Booking

The fixed annual price structure for AxioObserver Inverted Widefield usage allows unlimited booking of both the AxioObserver (Skou building) and the AxioObserver (Anatomy building). Within this structure, it is also possible for registered users to use the instruments for a quick sample check by booking a 15 min time slot.

Training Session

A training session is mandatory and is not included in the annual price. As the AxioObservers hold minor differences, the training session will be given to the most relevant AxioObserver and, if needed, supplemented with a free of charge, short add-on for the other AxioObserver.

Training and use

Tips and tricks

   

The image

  • Always locate and bring your sample in focus by looking through the occular before you play with software settings.

Specifications

Full equipment name Widefield Inverted Light Microscope, Zeiss
Objectives
  • 10x/0.25, Air, LongDistance A-Plan, coverslip thickness: 1 mm polystyrene
  • 40x/0.75, Air, EC Plan-Neofluar, coverslip thickness: 0.17 mm
  • 63x/1.40, Oil, Plan Apochromat, coverslip thickness: 0.17 mm
Filter qubes
  • Cy 5 (EX BP 640/30, BS FT 660, EM BP 690/50)
  • Cy3 (EX BP 550/25, BS FT 570, EM BP 605/70)
  • eGFP (EX BP 470/40, BS FT 495, EM BP 525/50)
  • Hoechst/DAPI (EX BP 390/40, BS FT 420, EM BP 450/40)
Light sources
  • Illuminator microLED (for brightfield applications)
  • Colibri 5 type RGB-UV (for fluorescence applications)
Detectors (cameras)
  • Axiocam 506 mono (for fluorescence applications)
  • Axiocam 105 color R2 (for brightfield applications) 
Heated stage No
Motorized stage No

Need help?

Come to our Open Office sessions to discuss your imaging needs or get advice on workflows, free of charge:

Tuesdays and Thursdays 13:30 -14:30

The Skou building, 1116-256