LSM 900, Laser Scanning Confocal, Zeiss

LSM 900, Laser Scanning Confocal, Zeiss

Inverted confocal laser scanning microscope Axio Observer 7 with AI sample finder and Real Time Controller Imaging systems

Specifications

Fluorescence illuminator: X-Cite Xylis XT720L and filters for DAPI, GFP, YFP, Cy3 and Cy5

Camera: Axiocam 305 mono

Detectors:

  • 2x PMT detectors
  • 1 Transmitted detector (No DIC filter)

Location:

The Skou building 1116, room 550

Objective Mag. / NA Medium Working Distance (mm)
Pln- Neofluar 10x/0,3 Air 5,2
Pln- Neofluar LD 20x/0,40 Air 7,9 (cover glass thickness 0-1,5 mm)
Pln- Neofluar 40x/1,30 Oil 0,21
Pln- Apo 63x/1,4 Oil 0,19
Fluar 20x/0,75 Air 0,6
Lasers      Type and Power      Selected dyes
405nm              Diode    DAPI, Hoechst, ...
488nm Diode GFP, AlexaFluor488
561nm Diode CY3, Alexa Fluor 546, 561, 594
640nm Diode CY5, Alexa Fluor 633, 647

Software

ZEN 3.5 system (Blue), incl. Experimental Designer, Tiles / Position and Connect modules.