Inverted confocal laser scanning microscope with Airyscan 2 detection array allowing from confocal to super-resolution imaging. Widefield camera for fast overview generation and AI sample finder. Overview scans from the Zeiss Slide scanner can be imported for detail scanning of previously identified ROIs.
Fluorescence illuminator: X-Cite Xylis XT720L and filters for DAPI, GFP, YFP, Cy3 and Cy5
Camera: Axiocam 305 mono
Detectors:
Objective | Mag./NA | Medium | Working Distance (mm) |
Pln- Neofluar | 10x/0,3 | Air | 5,2 |
Pln-Apo | 10x/0,45 | Air | 2,1 |
Pln- Neofluar LD | 20x/0,40 | Air | 7,9 (cover glass thickness 0-1,5 mm) |
Pln- Neofluar | 40x/1,30 | Oil | 0,21 |
Pln- Apo | 63x/1,4 | Oil | 0,19 |
Lasers | Type and Power | Selected dyes |
405nm | Diode | DAPI, Hoechst |
488nm | Diode | GFP, AlexaFluor488 |
561nm | Diode | CY3, Alexa Fluor 546, 561, 594 |
640nm | Diode | CY5, Alexa Fluor 633, 647 |
ZEN 3.5 system (Blue), incl. Experimental Designer, Tiles / Position and Connect modules.