LSM 710, Laser Scanning Confocal, Zeiss

Inverted Zeiss LSM710

Capabilities: Optical z-sectioning of various fluorophores in living specimens and fixed samples. Motorized XY stage, temperature control, 3D z-stacks, timelapse, stitching, multi-position

 

Detectors:

3 detectors 

1 Transmitted detector

OBJECTIVES
ObjectiveMag. / NAMediumContrastWorking Distance
Pln-Apo20x/0.8Air-0,55
C Apochromat 40x/1,2Water
DIC III  
0.28 at cover glass 0.17
Plan-Apochromat63x/1.4OilDIC III 0.19



 

Light sources

LasersType and PowerSelected dyes
405nmDiode, 30mWDAPI, Hoechst, ...
458nmArgon, 25mWCFP
488nmArgon, 25mWGFP, AlexaFluor488
514nmArgon, 25mWYFP
543 nm HeNe, 5mW RFP
633nmHeNe, 5mWCy5, AlexaFluor648

 

Location:

The Skou building 1116, room 455B

Start Up and Shut Down Procedures:

Booking

Visninger
LSM710 training video